Expression of prophybrotic markers TGF-β1, MMP-9 and FGFR in the kidneys of patients with urolithiasis during the development of chronic kidney disease

Napsheva A.M., Khotko D.N., Maslyakova G.N., Tarasenko А.I., Popkov V.M.

1) Saratov State Medical University n.a. V.I.Razumovsky, Saratov, Russia; 2) I.M. Sechenov First Moscow State Medical University of the Ministry of Health of Russia (Sechenov University), Moscow, Russia

Objective: to evaluate the expression of profibrotic markers TGF-β1, MMP-9 and FGFR in the epithelium of proximal renal tubules in patients with urolithiasis depending on the composition of the nodule, as well as on the degree of decrease in the glomerular filtration rate (GFR).
Material and methods: complex examination of 17 patients with urolithiasis was carried out. Cockcroft-Gault formula for determining the glomerular filtration rate was used to estimate renal function, according to the results of which all patients were divided into 3 groups depending on the stage of GFR. Percutaneous nephrolithotripsy (PNLT) was performed according to the standard technique using laser treatment. The physical and chemical composition of the nodule was determined by polarization microscopy. All patients underwent morphological and morphometric study of nephrobiopsy specimens obtained at creation of puncture passage during PNLT in patients with nephrolithiasis. Immunohistochemical study was performed using profibrotic marker Anti-TGF beta 1 antibody (1:500, Abcam, UK), Anti-MMP-9 antibody (1:500, Abcam, UK), Anti-FGFR-1 1:50, Abcam, UK).
Results. Analysis of the results of morphological study revealed a correlation only between morphological manifestations of CPN progression and expression of profibrotic marker TGF-β.
Conclusion. The increase in TGF-β expression is accompanied by more pronounced atrophic changes in the epithelium of proximal renal tubules, which indicates the triggering of tubulointerstitial fibrosis mechanisms and the role of this marker in the progression of chronic kidney disease in patients with urolithiasis.

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About the Authors

Corresponding author: D.N. Khotko – PhD, Senior Researcher at the Research Institute of Fundamental and Clinical Uronephrology, Saratov State Medical University n.a. V.I. Razumovsky, Saratov, Russia, e-mail: dnksar@list.ru

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